Plasmid

Part:BBa_I713015:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


LAA112


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4240
    Illegal BamHI site found at 5472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4306
    Illegal PstI site found at 750
    Illegal PstI site found at 6221
    Illegal PstI site found at 6245
    Illegal NgoMIV site found at 3076
    Illegal NgoMIV site found at 3430
    Illegal NgoMIV site found at 3590
    Illegal NgoMIV site found at 4208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 924
    Illegal BsaI.rc site found at 6146
    Illegal SapI.rc site found at 2006


Design Notes

First insert LacI with NcoI and BamHI.

Then insert EGFP with BamHI and HindIII.



Source

BBa_I713036

References